Greengate cloning
Web57 rows · GreenGate Cloning System(Kit # 1000000036 ) Depositing Lab: Jan Lohmann. The 56 plasmid GreenGate cloning kit can be used to create plant expression vectors containing several cassettes and generate multi …
Greengate cloning
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WebNow, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target organism. WebJan 20, 2024 · Here, we adapted the GreenGate cloning vectors to create two destination vectors showing strong marking of cell membranes in either the whole root or specifically in the lateral roots. This system can also be used in both embryos and whole seedlings. As proof of concept, we follow both gene expression and anatomy in Arabidopsis …
WebJan 4, 2024 · Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell-type specific trans-activation based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate … WebJul 19, 2024 · The driver lines were generated employing the fast and flexible GreenGate cloning system (Lampropoulos et al., 2013) but are compatible with any vector/transgenic line in which the expression of an effector is under the control of derivatives of the pOp promoter element (Moore et al., 1998). An important feature of our driver lines is the ...
WebNational Center for Biotechnology Information Webdestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression:
WebOct 29, 2024 · Cloning Greengate entry module construction. Primers with GreenGate overhangs were designed following the Greengate protocol. Control primers were designed at the same time for colony screens and sequencing. For a list of primers see Supplemental Table S1. Construction of destination vectors with red membrane included
WebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the … dick vitale 2022 ncaa bracket predictionsWebGreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS … city center impfenWebDec 19, 2013 · A) The GreenGate cloning system uses six different types of pUC19 based entry vectors into which the individual elements are inserted and a pGreen-IIS based destination vector. Magenta scissors represent BsaI recognition sites. In each GreenGate reaction, six modules are ligated between the left border (LB) and the right border (RB) … dick vitale bracket predictionWebGreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed Plasmids from Article ... dick vhilds guitar armyWebGreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors. All these make GreenGate versatile and applicable cloning system … city center houston store listWebOct 15, 2024 · One cloning kit, termed GreenGate, was designed to facilitate rapid cloning of . constructs using commonly used elements of plant transformation vectors [16]. Assembly . city center idaWebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were … dick vitale awesome baby college hoops