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Tpx2 phase

SpletTPX2 is an effector of microtubule nucleation in spindles, and functions with the 18 substrate tubulin by an unknown mechanism. Here, we show that TPX2 phase separates … SpletTheir mutation, by abolishing importin α interaction, causes TPX2 to become constitutively active in Xenopus M-phase egg extract. 3 Furthermore, TPX2 is a microtubule associated …

Structural insight into TPX2-stimulated microtubule assembly

Splet01. mar. 2009 · During the latter stages of the execution phase, actin–myosin-II drives the movement of apoptotic nuclear fragments into surface blebs (Lane et al., ... TPX2 siRNA: … Splet12. jan. 2001 · TPX2 was precipitated from M phase extract using affinity-purified antibodies against TPX2 (αTPX2) or rabbit IgG (control). Bound proteins were eluted sequentially with 2 M MgCl 2 (1st elution, lanes 3 and 4) and SDS sample buffer (2nd elution, lanes 5 and 6). Unbound (lanes 1 and 2) and bound (100-fold amount) proteins … darkening of tristram https://fchca.org

Phase Separation in Cell Division - ScienceDirect

Splet3 ; called TPX2 cds siRNA in this paper) has been described previously and specificity has been thoroughly demonstrated (5). This TPX2 cds siRNA targets a coding region of … SpletTPX2 (targeting protein for Xklp2)是一种定位于人类染色体20q11.1的微管相关蛋白，其表达水平与细胞周期密切相关，在细胞周期的G 1 ~S期开始被检测到，在细胞完成分裂后消 … SpletIn addition, we establish that TPX2 interacts with importin-β via dispersed, weak interactions. We show that interactions of both importin-α and -β with TPX2 inhibit its … darkening of the nipple at the tips of breast

Structural Basis of Aurora-A Activation by TPX2 at the

Category:InteractionofspindleassemblyfactorTPX2withimportins- inhibits …

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Tpx2 phase

Phase separation of TPX2 enhances and spatially coordinates.

Splet12. jun. 2024 · TPX2 is an effector of microtubule nucleation in spindles, and functions with the substrate tubulin by an unknown mechanism. Here, we show that TPX2 phase … Splet01. sep. 2024 · TPX2 is a key factor for branching microtubule nucleation, as it initiates this reaction by binding to microtubules and marking the branch site to recruit the other …

Tpx2 phase

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Splet15. jan. 2024 · Among the proteins that interact with and activate AURKA, some are well-established activators, such as Ajuba, TPX2, NEDD9 and PUM2. The LIM protein Ajuba efficiently stimulates AURKA autophosphorylation at Thr288 and increases kinase activity toward histone H3 in the late G2 phase [ 25 ]. SpletTPX2 is an effector of microtubule nucleation in spindles, and functions with the substrate tubulin by an unknown mechanism. Here, we show that TPX2 phase separates into a co …

Splet16. feb. 2024 · The AurkA kinase is a well-known mitotic regulator, frequently overexpressed in tumors. The microtubule-binding protein TPX2 controls AurkA activity, localization, and stability in mitosis. Non-mitotic roles of AurkA are emerging, and increased nuclear localization in interphase has been correlated with AurkA oncogenic potential. Still, the … Splet21. dec. 2015 · TPX2 is a microtubule-associated protein that is required for mitotic spindle assembly and function. TPX2 has a nuclear localization sequence (NLS) and is nuclear-localized during interphase; in mitosis it localizes to spindle microtubules, with an enrichment toward the spindle poles ( Figure 1 ). Figure 1 TPX2. Where does the name …

SpletKing MR, Petry S. Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation. Nat Commun 2024;11 (1):270. Abstract 2024 Thawani A, Stone HA, Shaevitz JW, Petry S. Spatiotemporal organization of branched microtubule networks. Elife 2024;8 Abstract 2024 SpletPhase separation of substrates and effectors is proposed to enhance biological reaction rates and efficiency. Targeting protein for Xklp2 (TPX2) is an effector of branching …

Splet03. nov. 2014 · Because TPX2's DNA damage response function is particularly evident during G1-phase (see ), we next determined the TPX2-dependent levels of H4K16ac at this cell cycle stage. To do so, we utilized the HeLa cell line expressing a doxycycline-inducible TPX2 miRNA and synchronized these cells with a double thymidine block [15] , [48] .

Splet13. mar. 2024 · TPX2 proteins are the main players determining the spatiotemporally dynamic nature of the MTs. However, how TPX2 members respond to abiotic stresses in poplar remains largely unknown. Herein, 19 TPX2 family members were identified from the poplar genome and analyzed the structural characteristics as well as gene expression … darkening of the neckSplet10. apr. 2024 · Bottom row, representative raw images of G2/M phase cells separated on PHATE coordinates. All images show mRNAs (white) in HeLa cells (DAPI in blue). ... G2/M genes: TOP2A, TPX2, UBE2C, HJURP ... darken hair naturally at homeSplet01. maj 2003 · It is found that TPX2 acts to suppress tubulin subunit off-rates during microtubule assembly and disassembly, thus allowing for the support of unprecedentedly slow rates of plus-end microtubules growth, and also leading to a dramatically reduced microtubULE shortening rate. Expand 38 PDF View 1 excerpt, cites background Save Alert bish fcSplet03. nov. 2014 · Overexpression of TPX2 alters the DAPI staining pattern of interphase cells and depletion of TPX2 constitutively decreases the levels of histone H4 acetylated at … bish fanSplet抗肿瘤药物靶标：周期蛋白依赖性激酶4/6 (CDK4/6) 细胞分裂失调引起的细胞增殖异常是肿瘤标志性特征之一。. 因此，识别并阻断细胞分裂的相关靶点是肿瘤治疗的一个重要方向 … bish fc twitterbish family originSplet16. feb. 2024 · The AurkA kinase is a well-known mitotic regulator, frequently overexpressed in tumors. The microtubule-binding protein TPX2 controls AurkA activity, localization, and … bish fan club